The Lionheart Klotho-Up Protocol builds gut and immunity health around nutrition, exercise, sleep and preventive care, with Lionheart technologies added as monitored adjuncts. The aim is balanced immune function, a healthier intestinal environment and fewer infections, not simply higher immune activity.
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20 Ways to Improve Gut and Immunity Health
| # | Way to improve gut and immunity health | Integration with Lionheart Klotho-Up |
|---|---|---|
| 1 | Make a Mediterranean-style diet the foundation | Center daily meals on vegetables, legumes, whole grains, fruit, nuts, olive oil and fish. Make nutrition coaching a core program service. |
| 2 | Meet an individualized fiber target | Generally aim for 22–34 g/day, increasing gradually. Track intake and tolerance; adjust for IBS or other digestive conditions. |
| 3 | Diversify plant foods | Rotate vegetables, beans, grains, nuts, seeds, herbs and fruits weekly. Use a food checklist to encourage variety without imposing a rigid “magic number.” |
| 4 | Include fermented foods if tolerated | Start with a small daily serving of unsweetened yogurt, kefir or fermented vegetables. Human research supports potential microbiome and inflammatory benefits, but does not establish fewer infections. |
| 5 | Feed beneficial microbes with prebiotic foods | Include oats, beans, onions, garlic, asparagus and other tolerated fiber sources. Introduce slowly when bloating is present. |
| 6 | Maintain adequate protein and calories | Include protein at each meal to support muscle and normal immune function. Individualize intake for age, kidney function and nutritional status alongside BodStim™ training. |
| 7 | Replace nutrient-poor foods | Reduce sugary drinks and frequent highly processed snacks; replace them with fiber-rich, nutrient-dense foods. Build this into the Klotho-Up meal plan. |
| 8 | Exercise aerobically every week | Progress toward 150 minutes/week of moderate activity, adapted to ability. Walking, cycling or swimming should accompany clinic sessions. |
| 9 | Preserve muscle through resistance training | Include strengthening at least twice weekly. Integrate supervised BodStim™ as an adjunct; assess strength and function without assuming EMS proves gut or immune improvement. |
| 10 | Protect sleep and circadian rhythm | Aim for approximately 7–9 hours, consistent wake times and evaluation of suspected sleep apnea. Track sleep within Klotho-Up. |
| 11 | Manage chronic stress | Add daily breathing, mindfulness, enjoyable activity and social connection. Brain Band™ or other modalities may be studied separately; their gut and immune effects remain unestablished. |
| 12 | Stay appropriately hydrated and support regular bowel habits | Individualize fluids, especially with heart or kidney disease. Pair hydration with fiber, movement and a regular toilet routine. |
| 13 | Correct nutrient deficiencies | Assess dietary adequacy and test selectively for deficiencies such as vitamin D, B12 or iron. Use targeted replacement rather than routine high-dose “immune” stacks. |
| 14 | Keep vaccinations current | Include a clinician review of vaccines appropriate to age, medical conditions and current guidance at enrollment. |
| 15 | Stop smoking and minimize alcohol | Offer cessation support and an alcohol-reduction plan. Track these exposures alongside other lifestyle measures. |
| 16 | Maintain oral and periodontal health | Include brushing, interdental cleaning and dental care. Add an oral-health review to the baseline assessment. |
| 17 | Address excess visceral fat and poor glucose control | Use sustainable nutrition and exercise changes; monitor waist circumference and HbA1c when appropriate. Avoid aggressive weight loss that sacrifices muscle in older participants. |
| 18 | Review medicines and use probiotics selectively | Avoid unnecessary antibiotics; review long-term acid suppression and other drugs with the prescriber. Choose probiotics for a specific indication and strain, with additional caution in immunocompromised patients. |
| 19 | Diagnose persistent digestive symptoms | Investigate ongoing diarrhea, constipation, pain, bleeding or unexplained weight loss. Treat identified disease rather than relying on a general “gut reset” or commercial microbiome score. |
| 20 | Evaluate Lionheart gut/Klotho interventions in a controlled research component | Study Second Brain™, relevant 240 Stimulus™ programs and Klotho-directed approaches with defined outcomes and appropriate oversight. Review Rejuvant/AKG, SNIP and KlothaMax individually; do not assume the stack improves immunity because Klotho rises. |
The fiber recommendation comes from the NIDDK. Fermented-food evidence includes a small randomized human study showing changes in microbiome diversity and inflammatory markers; the ideal amount and clinical benefits remain uncertain. Exercise targets follow CDC guidance. Adequate vitamins and minerals support immune function, particularly when correcting deficiencies; probiotic benefits depend on the product, strain and indication. Vaccine selection should follow current CDC age- and condition-specific guidance.
Suggested 12-Week Implementation
Daily nutrition, movement, sleep and symptom tracking; twice-weekly strengthening with supervised BodStim™ where appropriate; and clinician-directed treatment of deficiencies or digestive disease. Introduce optional supplements or experimental modalities one at a time so benefit and intolerance can be assessed.
Measuring Results
Measure results at baseline and week 12: a validated digestive symptom score, stool frequency/consistency, dietary fiber intake, sleep and functional performance. Track infection episodes, illness days and antibiotic use over 6–12 months. Use hs-CRP and Klotho as exploratory biomarkers: neither alone demonstrates improved immune protection, and a higher Klotho level should not substitute for clinical outcomes.
The Lionheart Klotho-Up Study is a one-year treatment protocol, so gut and immunity health are checked every 12 weeks and adjustments to therapy may be made.
Visit the Lionheart Klotho Wellness Program – Lionheart Klotho-Up Protocol IRB-Approved Study >
The Lionheart Klotho-Up Study Protocol combines a Klotho-favoring diet and supplements with Klotho-enhanced EMS exercise and targeted bioelectric stimulation in the basic mandatory arm. Options for bioelectrically enhanced peptides, Klotho exosomes, PRF, stromal vascular fraction and Klotho-expressing stem cells are available at licensed clinics outside the USA only. INVESTIGATIONAL RESEARCH.
Lionheart Second Brain™ Multimodal Protocol
Lionheart describes Second Brain as bioelectric signaling directed toward the gut–brain axis, and Second Brain Plus as combining stimulation with investigational biologics. Its website also links Viome testing. Those descriptions establish the program’s intended approach; they do not establish clinical effectiveness for every listed condition.
The following is the expanded protocol, with treatment settings finalized by Lionheart’s clinical and engineering teams.
| Component | Integration into Klotho-Up | What to evaluate |
|---|---|---|
| Personalized nutrition and microbiome support | Mediterranean-style diet, gradually increased fiber, diverse plants, tolerated fermented foods, adequate protein and clinician-reviewed Viome recommendations. | Digestive symptoms, bowel habits, dietary adherence and microbial functional changes. |
| Second Brain targeted bioelectric stimulation | Investigational gut-directed sessions using the Lionheart 240 Stimulus™ platform. Specify electrode placement, waveform, current, sequence, session duration and cumulative exposure in the study manual. | Whether treatment improves symptoms, bowel function, inflammation or immune endpoints beyond the foundational program. |
| BodStim™ muscle stimulation and exercise | Coordinate supervised BodStim™ with resistance exercise and aerobic activity. Record this exposure separately from Second Brain treatment. | Muscle function, metabolic health and possible associations with gut and immune changes. |
| Brain Band™ and gut–brain support | Optional research component alongside sleep optimization, stress management and breathing practice. | Stress, sleep, cognition and digestive symptom changes; avoid assuming a direct immune benefit. |
| Vagal/autonomic neuromodulation | Consider a separately defined research arm using an appropriate device and approved study procedures. Gut-directed stimulation should not automatically be described as vagus stimulation. | Autonomic measures and clinical outcomes; HRV can be supportive but is not a direct immunity measure. |
| Photobiomodulation — red/near-infrared light | Optional investigational adjunct. Document wavelength, irradiance, dose, treatment site and timing. | Tolerability and incremental benefit. Abdominal light exposure should not be assumed to deliver a therapeutic dose to intestinal tissue. |
| PEMF | Optional research adjunct with recorded field strength, frequency, treatment location and duration. | Safety and incremental effects on predefined outcomes; gut and immune rejuvenation remain hypotheses. |
| Klotho-Up supplement program | Review Rejuvant/AKG, SNIP, KlothaMax and any additional supplements for ingredients, doses, interactions and duplication. | Safety, adherence and exploratory Klotho changes alongside actual clinical outcomes. |
| Second Brain Plus biologics | Evaluate any proposed biologic, cell product, secretome or infusion system in a separately authorized investigational arm. | Product-specific safety, biological activity and clinical benefit. This is not a routine wellness addition. |
Suggested delivery: an initial clinical assessment followed by a 12-week intensive phase, with twice-weekly supervised visits as a proposed starting schedule and daily nutrition, movement, sleep and symptom tracking. Follow-up continues through 12 months for immune outcomes. There is currently no verified optimal Second Brain stimulation dose or schedule in the sources reviewed.
The combination is tested against a comparison group receiving the same nutrition, exercise and preventive care. That allows determination of the additional benefit of the Lionheart technologies.
How Gut Health Is Measured
| Measurement | What it contributes | Proposed timing |
|---|---|---|
| Viome Gut Intelligence™ | Stool RNA-based metatranscriptomics: microbial gene activity and proprietary gut-health scores, with food and wellness recommendations. | Baseline, month 6 and month 12; add week 12 if agreed with Viome for the research study. |
| Validated digestive symptom questionnaire | Use one consistent instrument, such as the Gastrointestinal Symptom Rating Scale; add IBS-SSS when studying participants with IBS. | Baseline, week 12, month 6 and month 12. |
| Bowel diary and Bristol Stool Form Scale | Stool frequency and form, straining, urgency, incomplete evacuation and rescue medication use. | Daily during defined assessment windows. |
| Fecal calprotectin, when indicated | Helps assess intestinal inflammation in the appropriate clinical context. It is not a universal microbiome or immune-age score. | Baseline and follow-up when symptoms or diagnosis justify testing. |
| Clinically indicated digestive testing | Celiac testing, pathogen testing, pancreatic elastase or other tests selected according to symptoms and examination. | As needed; avoid indiscriminate panels. |
| Research microbiome/metabolite assays | Optional shotgun sequencing, targeted stool short-chain fatty acids, bile acids and tryptophan metabolites to investigate mechanisms. | Baseline and selected follow-up visits. |
| Intestinal permeability assessment | A standardized sugar-permeability test may be considered for a mechanistic substudy. | Baseline and follow-up if justified. |
| Nutrition and quality of life | Fiber and protein intake, food tolerance, weight stability, GI-related quality of life and adherence. | Throughout the program. |
Viome currently describes 25+ gut-health scores and recommends retesting approximately every six months. Its results are intended for educational use rather than disease diagnosis. For research, the complete reports are preserved, along with the exact score definitions and algorithm versions used. Microbial RNA activity is not the same as directly measuring metabolite concentrations, intestinal permeability or immune function.
Viome Full Body Intelligence™ adds oral microbiome and cellular-health information. Its BioAge™ or InflammAging™ outputs should be reported under their own names, without treating them as equivalent to XPRIZE IMM-AGE or iAge.
XPRIZE Immune Assessments and Additional Biomarkers
The current public Finals framework requires improvement beyond personalized thresholds in two of three immune categories, assessed centrally:
| XPRIZE category | Assessment | Meaning |
|---|---|---|
| Immune-cell composition | IMM-AGE | A multidimensional assessment of immune aging, using standardized deep immune profiling. |
| Inflammatory status | iAge | An inflammatory-aging assessment involving centrally specified molecular measurements. |
| Response to challenge | Immune response/resilience assay or proxy — pending specification in the reviewed documents | Evaluates dynamic immune fitness rather than only resting inflammation. |
Lionheart Health collects PBMCs, plasma, serum and whole blood under XPRIZE procedures. Final assay details and collection schedules must follow the central laboratory’s instructions. A local CBC, cytokine panel or commercial age score cannot substitute for the official assessments.
Lionheart’s Additional Monitoring
| Additional panel | Suggested measures | Purpose |
|---|---|---|
| Basic clinical immune monitoring | CBC with differential; absolute lymphocyte and neutrophil counts. | Detect abnormalities and contextualize other results. |
| Expanded immune phenotyping | CD4/CD8 counts and ratio; naïve/memory T-cell subsets, B cells, NK cells and selected senescence/exhaustion markers. | Explore mechanisms; laboratory interpretation is essential, and these do not independently recreate IMM-AGE. |
| Inflammation | hs-CRP; a predefined research panel potentially including IL-6, TNF-α, IL-1β, IL-10 and CXCL9. | Track inflammatory patterns rather than assuming every cytokine should decrease. |
| Immune context | CMV status; immunoglobulins when clinically indicated. | Identify factors that influence immune profiles or recurrent infections. |
| Metabolic and safety monitoring | CMP, kidney/liver function, HbA1c and targeted nutrient testing. | Evaluate safety and contributors to impaired health. |
| Klotho-Up mechanisms | Circulating alpha-Klotho, with consistent assay and collection methods. | Exploratory target engagement — not proof of immune rejuvenation. |
| Real-world immune outcomes | Documented infections, illness days, antibiotic courses, hospitalizations and recovery duration. | Determine whether laboratory changes accompany meaningful health benefits. |
Measurement discipline matters. We standardize collection time and handling; record recent infections, vaccinations, antibiotics, medication changes and exercise exposures; and prespecify the primary outcomes. We follow the official XPRIZE baseline and follow-up schedule and add Lionheart’s week-12 gut assessments with strict adherence.
The strongest evidence package from this study is intended to show better digestive function, favorable microbiome activity changes and improvement in official immune endpoints, supported by fewer or shorter illnesses. A change in Viome scores, Klotho or hs-CRP alone would support further investigation designed to establish whether Second Brain has rejuvenated immunity.